EXPLORATION OF INTESTINAL MICROBIOTA OF BREASTFEEDING MOTHERS WITH THE HABIT OF DRINKING DANGKE WATER AS A LOCAL FOOD OF THE ENREKANG COMMUNITY
DOI:
https://doi.org/10.12873/461citrakesumasariPalabras clave:
dangke water; breastfeeding mothers; gut microbiota; sequencing; local foodResumen
Background:The people of Enrekang Regency, especially in Curio District, have a habit of consuming dangke water (local cheese-making waste), including breastfeeding mothers. There has not been much research related to the habit of drinking dangke water on gut health. Objective: To describe the gut microbiota profile of breastfeeding mothers who have a habit of consuming dangke water. Method: Descriptive study with a cross-sectional design, a sample size of 42 breastfeeding mothers. The group that consumed dangke water (intervention) was 33 people from Curio District and 9 people from the non-consumption group (control) from Kabere District. Direct visits were conducted to the intervention group to ensure drinking 150ml/day for 3 months. The data collected included subject characteristics, nutritional intake, and fecal samples. Samples were analyzed using 16S rRNA gene sequencing at the HUM-RC Unhas Laboratory and then analyzed bioinformatics wf-metagenomics Sequencing Report. Results:A total of 42 breastfeeding mothers participated in this study, predominantly aged 30–39 years (45.2%), with a high school education (47.6%), and working as housewives (54.8%). Alpha-diversity analysis showed that the intervention group had a higher species richness than the control group (richness 77.00 ± 17.95 vs 55.73 ± 16.51). At the phylum level, Bacillota was dominant in both groups (82.86% vs 85.28%), but the intervention group showed a higher proportion of Bacteroidota (7.21% vs 6.27%).At the species level, the intervention group had higher numbers of several good bacteria that are important for gut health., namely Faecalibacterium prausnitzii (13.32% vs 12.37%), Agathobacter rectalis (7.14% vs 5.82%), Blautia wexlerae (5.54% vs 4.60%), and Segatella copri (5.60% vs 4.81%). Conclusion:Consumption of dangke water is associated with increased richness, total microbes, and butyrate-producing species, indicating a more favorable gut microbiota profile in breastfeeding mothers compared to the control group.
Referencias
1. World Health Organization. World Breastfeeding Week 2023. Jakarta: WHO Indonesia; 2023.
2. Agustina R, Rianda D, Lasepa W, Birahmatika FS, Stajic V, Mufida R. Nutrient intakes of pregnant and lactating women in Indonesia and Malaysia: Systematic review and meta-analysis. Frontiers in Nutrition. 2023 Mar 30;10:1030343. DOI: https://doi.org/10.3389/fnut.2023.1030343
3. Rachmadiani AP, Nadhiroh SR. The nutritional status of breastfeeding mothers and breastfeeding adequacy in Indonesia: a systematic review. J Maternal Child Health. 2024;9(4):450–61. DOI: https://doi.org/10.26911/thejmch.2024.09.04.02
4. Uswatun HS, Citrakesumasari, Indarty AM. Relationship between baby weight and alpha-lactalbumin content in breast milk in Makassar. Glob Acad J Med Sci. 2019;1(2):33–6.
5. Sumule I, Citrakesumasari, Daud NA, Salam A, Indriasari R, Ibrahm E. Differences in oleic acid levels in matured milk of breastfeeding mothers with normal and chronic energy deficiency. Int J Papier Adv Sci Rev. 2020;1(2):53–63. DOI: https://doi.org/10.47667/ijpasr.v1i2.50
6. Muhrifan A, Citrakesumasari, Djide N, Sirajuddin S, Jafar N, Naiem F. Differences of oleic acid levels in breast milk of lactating mothers with chronic energy deficiency (CED) and normal status. J Sci Res Med Biol Sci. 2020;1(2):161–70. DOI: https://doi.org/10.47631/jsrmbs.v1i2.142
7. Asmi AD. Fat intake with oleic acid levels in breast milk of breastfeeding mothers (6–24 months) in Makassar [dissertation]. Makassar: Hasanuddin University; nd
8. Awaru AFT, Russeng SS. Factors associated with protein concentration in breastmilk of women in Makassar, Indonesia. Malays J Med Health Sci. 2023;19.
9. Hatta W, Yuliati FN, Ningrum EM. Physicochemical characteristics of whey dangke powder with carrageenan variation. J Animal Sciences. 2019;29(3):249–58. DOI: https://doi.org/10.21776/ub.jiip.2019.029.03.07
10. Feng C, Tian L, Hong H, Wang Q, Zhan X, Luo Y, et al. In vitro gut fermentation of whey protein hydrolyzate: modulation of infant gut microbiome. Nutrients. 2022;14(7):1374. DOI: https://doi.org/10.3390/nu14071374
11. Zubeldia-Varela E, Barker-Tejeda T, Obeso D, Villaseñor A, Barber D, Pérez-Gordo M. Microbiome and allergies: new insights. J Investig Allergol Clin Immunol. 2022;32(5):327–44. DOI: https://doi.org/10.18176/jiaci.0852
12. Gebrayel P, Nicco C, Khoury N, et al. Microbiota medicine: toward clinical revolution. J Transl Med. 2022;20:1–20. DOI: https://doi.org/10.1186/s12967-022-03296-9
13. Tremaroli V, Bäckhed F. Functional interactions between gut microbiota and host metabolism. Nature. 2012;489:242–9. DOI: https://doi.org/10.1038/nature11552
14. Chen Y, Zhou J, Wang L. Role and mechanism of gut microbiota in human disease. Front Cell Infect Microbiol. 2021;11:625913. DOI: https://doi.org/10.3389/fcimb.2021.625913
15. Januszkiewicz E, Łoś-Rycharska E, Lis K, et al. Importance of the gut microbiome in allergic diseases. Allergol Pol. 2023;10(3):202–9. DOI: https://doi.org/10.5114/pja.2023.131711
16. Wu S, Bhat ZF, Gounder RS, Ahmed IA, Al-Juhaimi FY, Ding Y, et al. Effect of dietary protein and processing on gut microbiota: a systematic review. Nutrients. 2022;14(3):676. DOI: https://doi.org/10.3390/nu14030453
17. Syah SPS. Isolation and characterization of lactic acid bacteria from dangke and their potential for the production of fermented whey beverages. Pekalongan: Nasya Expanding Management; 2022.
18. Jackson JG, Janszen DB, Lonnerdal B, Lien EL, Pramuk KP, Kuhlman CF. A multinational study of α-lactalbumin concentrations in human milk. J Nutr Biochem. 2004;15(9):517–21. DOI: https://doi.org/10.1016/j.jnutbio.2003.10.009
19. Butts CA, Hedderley DI, Herath TD, et al. Human milk composition and dietary intakes among breastfeeding women in New Zealand. Nutrients. 2018;10(9):1231. DOI: https://doi.org/10.3390/nu10091231
20. Sofya SNY, Citrakesumasari, Hidayanthy H, Thaha R, Balqis, Zainal. Effect of olive oil intake on oleic acid in breast milk of mothers in Indonesia. J Chem Health Risks. 2024;14(1):1854–9.
21. Supardin TD, Citrakesumasari, Khuzaimah A, Djafar N, Asfar M, Syria. Effect of extra virgin olive oil on oleic acid and fat intake in exclusive breastfeeding mothers. J Chem Health Risks. 2024;14(1):2362–8.
22. Abidin SW, Citrakesumasari, Bahar B, Djafar N, Hidayanti H, Hadju V. Effect of EVOO on fat mass in breastfeeding mothers. J Educ Health Promot. 2024;13:127. DOI: https://doi.org/10.4103/jehp.jehp_907_23
23. Wastyk HC, Perelman D, Fragiadakis GK, et al. Gut-microbiota-targeted diets modulate human immune status. Cell. 2021;184(16):4137–4153.e14. DOI: https://doi.org/10.1016/j.cell.2021.06.019
24. Kato K, Ishida S, Aoki R, et al. Fermented foods and gut microbiota: a cross-sectional study in Japanese adults. Nutrients. 2022;14(3):540.
25. Melini F, Melini V. Role of fermented foods in gut microbiota composition and health: recent evidence. Foods. 2022;11(18):2759.
26. Morrison KE, Jašarević E, Howard AG, et al. Dietary patterns shape gut microbial communities and metabolic health in women. Gut Microbes. 2022;14(1):2057772.
27. Grootaert C, Verstraeten V, Courtin CM, et al. Factors supporting short-chain fatty acid production: recent insights. Nutrients. 2023;15(2):320.
28. Zhang T, Li Q, Cheng L, et al. Regional dietary habits drive gut microbiome structure: a multicentre study. Microbiome. 2023;11:57. DOI: https://doi.org/10.1186/s40793-023-00516-7
29. Jin M, Lu J, Zhao J, et al. Traditional fermented foods modulate gut microbiota and immunity: a systematic review. Mol Nutr Food Res. 2024;68(1):e2300456.
30. Cortés-Macías E, Selma-Royo M, Martínez-Costa C, et al. Maternal gut microbiota and breastfeeding: current evidence. Nutrients. 2023;15(4):1001.
Descargas
Publicado
Número
Sección
Categorías
Licencia
Derechos de autor 2026 Nutrición Clínica y Dietética Hospitalaria
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.
Los lectores pueden utilizar los textos publicados de acuerdo con la definición BOAI (Budapest Open Access Initiative)
Cómo citar
